Insulin-like growth factor I expression by tumors of neuroectodermal origin with the t(11;22) chromosomal translocation. A potential autocrine growth factor Academic Article uri icon

Overview

MeSH Major

  • Chromosomes, Human, Pair 11
  • Chromosomes, Human, Pair 22
  • Insulin-Like Growth Factor I
  • Neuroblastoma
  • Neuroectodermal Tumors, Primitive, Peripheral
  • Receptors, Cell Surface

abstract

  • Expression of insulin-like growth factor I (IGF-I) mRNA by some tumor cell lines of neuroectodermal origin has been described. To further explore the significance of IGF-I mRNA expression in these tumors, a more extensive analysis was performed. Most (9 of 10) neuroectodermal tumor cell lines with a t(11;22) translocation (primitive neuroectodermal tumor [PNET], Ewing's sarcoma, esthesioneuroblastoma) expressed IGF-I mRNA, whereas 0 of 15 cell lines without the translocation (PNET, neuroblastoma) expressed IGF-I. Furthermore, inasmuch as all neuroblastoma (12 of 12) cell lines examined expressed IGF-II RNA, the pattern of IGF expression could distinguish between these closely related tumors. CHP-100, a PNET cell line with the t(11;22) translocation, was shown to secrete both IGF-I protein and an IGF binding protein, IGFBP-2. This cell line also expressed the type I IGF receptor mRNA, and blockade of this receptor by a monoclonal antibody (alpha IR3) inhibited serum-free growth. These data demonstrate that IGF-I expression is a property of neuroectodermal tumors with a t(11;22) translocation and that interruption of an IGF-I autocrine loop inhibits the growth of these tumor cells.

publication date

  • December 1990

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC329812

PubMed ID

  • 2174908

Additional Document Info

start page

  • 1806

end page

  • 14

volume

  • 86

number

  • 6