Diversity in junctional sequences associated with the common human V gamma 9 and V delta 2 gene segments in normal blood and lung compared with the limited diversity in a granulomatous disease. Academic Article Article uri icon

Overview

MeSH

  • Adult
  • Antibodies, Monoclonal
  • Base Sequence
  • DNA
  • Female
  • Flow Cytometry
  • Gene Expression
  • Genetic Variation
  • Humans
  • Male
  • Molecular Sequence Data
  • Phenotype
  • RNA, Messenger
  • Receptors, Antigen, T-Cell, gamma-delta
  • Sequence Homology, Nucleic Acid

MeSH Major

  • Lung
  • Receptors, Antigen, T-Cell
  • Sarcoidosis
  • T-Lymphocytes

abstract

  • The T cell receptor (TCR) junctional regions (N regions) of the common human V gamma 9 and V delta 2 gene segments were sequenced from the blood and lung of normal individuals (195 transcripts) and a group of individuals with sarcoidosis (220 transcripts), a granulomatous disease in which increased numbers of V gamma 9+ gamma/delta + T cells are often observed. In normal individuals, the vast majority (86%) of blood V gamma 9 transcripts used the J gamma P gene segment. In contrast to this restriction of J region usage, there was a large diversity of the junctional region, with less than 20% of blood V gamma 9 junctional regions showing identical sequences for any one normal individual. For the blood V delta 2 transcripts in normal individuals, there was restriction of J region usage, with 93% using J delta 1. The junctional regions were even more diverse than for V gamma 9, with a unique sequence observed in each transcript examined. Compared with blood, sequences from the normal lung showed a small increase in identical junctional regions, particularly in one individual where 46% of V gamma 9 transcripts examined were identical, suggesting a response of some gamma/delta T cells to antigens found in the lung in the normal state. In marked contrast to normals, some individuals with sarcoidosis had large numbers of V gamma 9 transcripts, as well as V delta 2 transcripts, sharing identical sequences. For V gamma 9 blood transcripts, two individuals showed 84 and 56% of junctional region sequences to be identical, respectively. Similarly, blood V delta 2 transcripts showed 43, 33, and 25% identical junctional region sequences in three individuals. In the sarcoid patient with the most striking over-representation of blood V gamma 9 junctional sequences, lung V gamma 9 transcripts showed increased (67%) use of the same junctional region sequence as in blood. This limited diversity of TCR junctional regions among some individuals with sarcoidosis suggests a response from specific stimuli, possibly antigenic, and that gamma/delta T cells may play a specific role in granuloma formation in sarcoidosis, as has been suggested in other granulomatous diseases.

publication date

  • July 1, 1990

has subject area

  • Adult
  • Antibodies, Monoclonal
  • Base Sequence
  • DNA
  • Female
  • Flow Cytometry
  • Gene Expression
  • Genetic Variation
  • Humans
  • Lung
  • Male
  • Molecular Sequence Data
  • Phenotype
  • RNA, Messenger
  • Receptors, Antigen, T-Cell
  • Receptors, Antigen, T-Cell, gamma-delta
  • Sarcoidosis
  • Sequence Homology, Nucleic Acid
  • T-Lymphocytes

Research

keywords

  • Comparative Study
  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2188169

PubMed ID

  • 2141626

Additional Document Info

start page

  • 169

end page

  • 181

volume

  • 172

number

  • 1