Human immunodeficiency virus infection is efficiently mediated by a glycolipid-anchored form of CD4 Academic Article Article uri icon


MeSH Major

  • Cell Cycle Proteins
  • DNA-Activated Protein Kinase
  • DNA-Binding Proteins
  • Protein-Serine-Threonine Kinases
  • Recombination, Genetic
  • Tumor Suppressor Proteins


  • Two broad roles have been revealed for the CD4 molecule. It serves as a receptor for both class II major histocompatibility complex molecules and human immunodeficiency virus (HIV). Upon binding class II major histocompatibility molecules, CD4 functions to enhance T-cell activation. By binding to CD4, HIV gains entry into the cell. We have used a chimeric molecule of CD4 and lymphocyte function-associated antigen 3 (LFA-3), CD4PI, which lacks a membrane-spanning domain and is instead anchored in the membrane by linkage to glycosyl-phosphatidylinositol. To further define the structural attributes of viral receptors, and specifically those of CD4 required for HIV infection, we have expressed CD4PI and CD4 in a human T-cell line, HSB-2. We find that CD4PI is able to mediate infection of these cells by HIV with similar, if not greater efficiency, compared with wild-type CD4. Thus the membrane-spanning region of CD4 is not required for HIV infection, and a lipid-anchored protein can serve as a viral receptor.

publication date

  • August 6, 1990



  • Academic Article


Digital Object Identifier (DOI)

  • 10.1073/pnas.87.13.5001

PubMed ID

  • 2142306

Additional Document Info

start page

  • 5001

end page

  • 5


  • 87


  • 13