Detection of preferential NRAS mutations in human male germ cell tumors by the polymerase chain reaction Academic Article uri icon

Overview

MeSH Major

  • African Americans
  • Colorectal Neoplasms
  • Communication
  • European Continental Ancestry Group
  • Mass Screening
  • Physician-Patient Relations
  • Practice Patterns, Physicians'

abstract

  • We have studied 31 male germ cell tumors (GCTs) for probable mutations in codons 12, 13, and 61 of HRAS, KRAS, and NRAS oncogenes using the polymerase chain reaction. Twenty of the thirty-one tumors exhibited NRAS gene mutations, 14 in codon 61, and six in codon 12, whereas no mutations were detected in HRAS and KRAS genes. The NRAS mutations were equally prevalent in seminomatous and nonseminomatous GCTs. Thus 13 of 22 seminomas, six of seven embryonal carcinomas, and one of two mixed tumors exhibited mutations. Two non-seminomatous tumors (an embryonal carcinoma and a yolk sac/teratoma) had mutations in both codons 12 and 61. The high frequency of NRAS mutations observed in the present study suggests that NRAS gene products may play an important role in growth regulatory functions of premalignant and malignant germ cells.

publication date

  • January 1990

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1002/gcc.2870010307

Additional Document Info

start page

  • 228

end page

  • 32

volume

  • 1

number

  • 3