Glycolipid precursors for the membrane anchor of Trypanosoma brucei variant surface glycoproteins. I. Can structure of the phosphatidylinositol-specific phospholipase C sensitive and resistant glycolipids. Academic Article uri icon

Overview

MeSH

  • Animals
  • Carbohydrate Sequence
  • Chromatography, Gel
  • Chromatography, High Pressure Liquid
  • Chromatography, Thin Layer
  • Glycosylphosphatidylinositols
  • Inositol
  • Kinetics
  • Molecular Sequence Data
  • Polysaccharides

MeSH Major

  • Glycolipids
  • Membrane Lipids
  • Phosphatidylinositols
  • Trypanosoma brucei brucei
  • Type C Phospholipases
  • Variant Surface Glycoproteins, Trypanosoma

abstract

  • A number of eukaryotic surface glycoproteins, including the variant surface glycoproteins of Trypanosoma brucei, are synthesized with a carboxyl-terminal hydrophobic peptide extension that is cleaved and replaced by a complex glycosyl-phosphatidylinositol (GPI) membrane anchor within 1-5 min of the completion of polypeptide synthesis. The rapidity of this carboxyl-terminal modification suggests the existence of a prefabricated precursor glycolipid that can be transferred en bloc to the polypeptide. We have reported the purification and partial characterization of a candidate precursor glycolipid (P2) and of a compositionally similar glycolipid (P3) from T. brucei (Menon, A. K., Mayor, S., Ferguson, M. A. J., Duszenko, M., and Cross, G. A. M. (1988) J. Biol. Chem. 263, 1970-1977). The primary structure of the glycan portions of P2 and P3 have now been analyzed by a combination of selective chemical fragmentation and enzymatic glycan sequencing at the subnanomolar level. The glycans were generated by deamination, NaB3H4 reduction, and dephosphorylation of glycolipids purified from different trypanosome variants. Glycan fragments derived from biosynthetically labeled glycolipids were also analyzed. The cumulative data strongly suggest that P2 and P3 contain ethanolamine-phosphate-Man alpha 1-2Man alpha 1-6Man alpha 1-GlcN linked glycosidically to an inositol residue, as do all the GPI anchors that have been structurally characterized. The structural similarities suggest that GPI membrane anchors are derived from common precursor glycolipids that become variably modified during or after addition to newly synthesized proteins.

publication date

  • April 15, 1990

has subject area

  • Animals
  • Carbohydrate Sequence
  • Chromatography, Gel
  • Chromatography, High Pressure Liquid
  • Chromatography, Thin Layer
  • Glycolipids
  • Glycosylphosphatidylinositols
  • Inositol
  • Kinetics
  • Membrane Lipids
  • Molecular Sequence Data
  • Phosphatidylinositols
  • Polysaccharides
  • Trypanosoma brucei brucei
  • Type C Phospholipases
  • Variant Surface Glycoproteins, Trypanosoma

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 2138614

Additional Document Info

start page

  • 6164

end page

  • 6173

volume

  • 265

number

  • 11