Nucleotide sequence of a human monoclonal anti-idiotypic antibody specific for a rabies virus-neutralizing monoclonal idiotypic antibody reveals extensive somatic variability suggestive of an antigen-driven immune response Academic Article uri icon

Overview

MeSH Major

  • Antibodies, Anti-Idiotypic
  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Genes, Immunoglobulin
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Variable Region
  • Rabies virus

abstract

  • We previously described the isolation and characterization of a human monoclonal anti-idiotypic antibody (Ab2) isolated from EBV-transformed human PBL after immunization with rabies vaccine. The present study concerns the molecular characteristics of the Ab2 and the germ-line elements that gave rise to it. The H chain of this antibody derives from the small VHV family of human V region gene segments. Parallel studies on the germ-line VHV gene isolated from the same individual revealed that the expressed molecule contains 19 nucleotide differences in the VH gene segment. The D segment of Ab2 could have arisen by a D to D fusion; the J segment is a JH6. Extensive somatic variation evident in the H chain variable region of this naturally arising monoclonal anti-idiotypic antibody suggests that this Ab2, the product of a CD5+ B cells, was the consequence of an Ag-driven immune response.

publication date

  • January 1990

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 2108216

Additional Document Info

start page

  • 2835

end page

  • 9

volume

  • 144

number

  • 7