Mediation of virion penetration into vascular cells by association of basic fibroblast growth factor with herpes simplex virus type 1. Academic Article uri icon

Overview

MeSH

  • Animals
  • Cell Line
  • Kinetics
  • Phosphorylation
  • Receptors, Fibroblast Growth Factor
  • Recombinant Proteins

MeSH Major

  • Fibroblast Growth Factor 2
  • Receptors, Cell Surface
  • Simplexvirus
  • Virion

abstract

  • Herpes simplex virus type-1 (HSV-1) is a ubiquitous pathogen that is associated with considerable morbidity in the general population. Although it is known that the virion uses a basic fibroblast growth factor (FGF) receptor to penetrate vascular cells, it is not known how the viral particle recognizes and binds to this cell surface protein. Here we report that an immunoreactive basic FGF-like protein is associated with the viral particle and that this association appears responsible for viral uptake. Accordingly, HSV-1 infection of Swiss 3T3 cells stimulates the tyrosine phosphorylation of the specific substrate that characterizes the initial cellular response to basic FGF. Antibodies to basic FGF prevent this phosphorylation and inhibit HSV-1 uptake. Because no basic FGF sequence is found in the HSV-1 genome, a model for the infection for some target cells is presented whereby the viral particle uses host cell-derived basic FGF to ensure subsequent infectivity of newly replicated virus.

publication date

  • November 22, 1990

has subject area

  • Animals
  • Cell Line
  • Fibroblast Growth Factor 2
  • Kinetics
  • Phosphorylation
  • Receptors, Cell Surface
  • Receptors, Fibroblast Growth Factor
  • Recombinant Proteins
  • Simplexvirus
  • Virion

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1038/348344a0

PubMed ID

  • 2174511

Additional Document Info

start page

  • 344

end page

  • 346

volume

  • 348

number

  • 6299