Gene Amplification in Gastric and Esophageal Adenocarcinomas Academic Article uri icon

Overview

MeSH Major

  • Adenocarcinoma
  • Esophageal Neoplasms
  • Gene Expression Regulation, Neoplastic
  • Oncogenes
  • Stomach Neoplasms

abstract

  • In a United States series of 28 gastric/esophageal adenocarcinomas of poorly to moderately differentiated histopathology, we detected gene amplification in 21% of the tumors. Using the modified DNA in-gel renaturation assay to detect down to 7-8 copies of amplified DNA sequences, we identified 3 gastric tumors with amplified DNA sequences and confirmed by Southern hybridization analysis that HER-2/neu was amplified greater than 5-fold in these specimens. Immunohistochemical staining of tumor tissue sections with p185 HER-2/neu antibodies demonstrated that only the 3 gastric adenocarcinomas with corresponding HER-2/neu gene amplification displayed membrane immunoreactivity. Amplification of c-met was identified in 2 tumors, and this was the first study to assay for multiple copies of this protooncogene in fresh gastric tumor tissues. Amplification of c-erbB was observed in one tumor, and no evidence for amplification of int-2 was obtained in this series of adenocarcinomas.

publication date

  • October 1990

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 2400999

Additional Document Info

start page

  • 6417

end page

  • 22

volume

  • 50

number

  • 19