Interchangeable RNA polymerase I and II enhancers Academic Article uri icon

Overview

MeSH Major

  • Enhancer Elements, Genetic
  • RNA Polymerase I
  • RNA Polymerase II
  • Saccharomyces cerevisiae

abstract

  • The RNA polymerase I (pol I) enhancer of Saccharomyces cerevisiae contains at least three elements commonly associated with RNA polymerase II (pol II) enhancers, binding sites for the transcriptional activators general regulatory factor 2 and autonomously replicating sequence-binding factor I, and a thymidine-rich element. When the particular form of the thymidine-rich element found in the pol I enhancer was placed in front of a pol II promoter, transcription was stimulated 43-fold, comparable to the effect of a powerful pol II activator such as Gal4. Conversely, when two copies of a thymidine-rich element from a pol II enhancer were placed upstream of a pol I promoter, transcription was stimulated 38-fold. This functional reciprocity of pol I and II enhancers may reflect similarities in the mechanisms of transcriptional activation. The pol I enhancer also contains an element that appears to be pol I-specific and prevent the activation of pol II.

publication date

  • November 21, 1990

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC54923

PubMed ID

  • 2236033

Additional Document Info

start page

  • 8202

end page

  • 6

volume

  • 87

number

  • 21