Molecular cloning of a human immunoglobulin heavy chain variable (V H ) region with anti-myelin-associated glycoprotein activity Academic Article Article uri icon


MeSH Major

  • Antibodies, Anti-Idiotypic
  • Gangliosides
  • Immunoglobulin M
  • Myelin-Associated Glycoprotein


  • A cDNA clone that encodes the heavy chain variable region (VH) of an IgM M-protein with anti-myelin-associated glycoprotein (MAG) activity secreted by chronic lymphocytic leukemia cells (B-C11) from a patient with peripheral neuropathy was cloned and sequenced. The JH region was identical to the germline JH4 sequence except for deletion of a thymidine residue at the site of D-JH recombination, and the D region showed greatest homology to DM2. Sequence analysis of the VH region revealed greatest homology to VH26, a member of the VH3 gene family, but homology was only 83.7% over 326 bases, suggesting that it was derived from as yet an unidentified member of the VH3 gene family.

publication date

  • January 1990



  • Academic Article


Digital Object Identifier (DOI)

  • 10.1016/0165-5728(90)90117-6

PubMed ID

  • 1688442

Additional Document Info

start page

  • 35

end page

  • 41


  • 26


  • 1