Molecular characteristics of a human anti-idiotypic B cell clone
Gene Expression Profiling
We previously described the isolation and characterization of a naturally arisen human CD5 + EBV-transformed B cell clone (EBV 383), that was derived from a donor after immunization with rabies vaccine. The heavy chain of the anti-idiotypic antibody (Ab 2 383), that is secreted by EBV 383, derives from the small V(H)V family of human variable region gene segments. The D segment of this Ab 2 383 could have arisen by an inverted complementary D to D fusion, the J segment is a J(H)6. Parallel studies on the germline V(H)V gene, isolated from the same donor, revealed extensive somatic variation in the heavy chain variable region of Ab 2 383. This suggested that Ab 2 383 was the product of an antigen driven immune response.