Detection of fos oncogene products by monoclonal antibody FO-120 in lymphoproliferative disorders Academic Article Article uri icon

Overview

MeSH Major

  • Antigens, CD38
  • Apoptosis
  • Aptamers, Nucleotide
  • Drug Carriers
  • Drug Delivery Systems
  • Membrane Glycoproteins
  • Multiple Myeloma

abstract

  • We investigated the expression of fos oncogene proteins in lymphoproliferative disorders, using a monoclonal antibody (FO-120) that was prepared against a synthetic oligopeptide of fos protein (amino acid sequence from 127 to 152). Although peripheral blood leukocytes were rarely positive for FO-120, they were transiently stained after lectin (PHA) stimulation. After culture with IL-2 for 1 or 2 weeks, less than 40% of the lymphocytes weakly reacted with FO-120, whereas strongly positive cells were detected in more than 70% of cells in half the T-cell lines established from preleukemic state of adult T-cell leukemia (pre-ATL) and all of ATL derived T-cell lines. All in vivo specimens of non-Hodgkin's malignant lymphomas, except for one case of T-cell lymphoma were also strongly positive. In addition, the extent of the antibody reactivity correlated with the histopathological grade of malignancy in B-cell lymphoma. The reactivity to most AILD-IBL lesions overlapped with that to T-lymphomas, and could be distinguished from that to reactive lesions. FO-120 appears to be a useful tool for detecting early neoplastic changes in lymphoproliferative disorders.

publication date

  • January 1989

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1016/0145-2126(89)90010-6

PubMed ID

  • 2514320

Additional Document Info

start page

  • 1025

end page

  • 33

volume

  • 13

number

  • 11