Morphologic characterization of alveolar macrophages from subjects with occupational exposure to inorganic particles. Academic Article uri icon

Overview

MeSH

  • Adult
  • Asbestos
  • Bronchoalveolar Lavage Fluid
  • Cell Nucleus
  • Coal
  • Humans
  • Middle Aged
  • Organelles
  • Silicon Dioxide

MeSH Major

  • Air Pollutants, Occupational
  • Dust
  • Macrophages
  • Pulmonary Alveoli

abstract

  • Alveolar macrophages recovered by bronchoalveolar lavage from 43 nonsmoking or greater than 5-yr ex-smoking subjects with occupational exposure to inorganic particles (asbestos, n 1/2 19; silica, n 1/2 10; coal, n 1/2 14) were evaluated by light microscopy and transmission and scanning electron microscopy to determine the morphologic changes resulting in these cells from chronic inorganic particulate inhalation. Alveolar macrophages from dust-exposed subjects, including those who had been free of exposure to particles for more than 1 yr, contained particles of higher proportion than did those of normal unexposed subjects. Most of these particles were located within phagolysosomes. The frequency of multinucleated alveolar macrophages was significantly higher in the dust-exposed groups. Ultrastructural studies showed alterations of the morphologic aspects of the surfaces of alveolar macrophages from the dust-exposed subjects, including increased numbers of rufflings, filopodia, pinocytotic vesicles, subplasmalemmal linear densities, and increased frequency of macrophage-macrophage and macrophage-lymphocyte interactions. Furthermore, the numbers of lysosomes were significantly increased in alveolar macrophages from the dust-exposed subjects. Together, these morphologic changes are consistent with the sequelae of phagocytosis, and they emphasize both the role of alveolar macrophages in eliminating inorganic particles from the alveolar spaces and the consequences this role has in alveolar macrophage activation.

publication date

  • December 1989

has subject area

  • Adult
  • Air Pollutants, Occupational
  • Asbestos
  • Bronchoalveolar Lavage Fluid
  • Cell Nucleus
  • Coal
  • Dust
  • Humans
  • Macrophages
  • Middle Aged
  • Organelles
  • Pulmonary Alveoli
  • Silicon Dioxide

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1164/ajrccm/140.6.1674

PubMed ID

  • 2557785

Additional Document Info

start page

  • 1674

end page

  • 1685

volume

  • 140

number

  • 6