Glutathione deficiency in the epithelial lining fluid of the lower respiratory tract in idiopathic pulmonary fibrosis. Academic Article Article uri icon

Overview

MeSH

  • Adult
  • Antioxidants
  • Cell Count
  • Epithelium
  • Female
  • Humans
  • Male
  • Middle Aged
  • Oxidation-Reduction

MeSH Major

  • Bronchoalveolar Lavage Fluid
  • Glutathione
  • Pulmonary Alveoli
  • Pulmonary Fibrosis

abstract

  • Glutathione (L-gamma-glutamyl-L-cysteinyl-glycine, GSH), a sulfhydryl-containing tripeptide produced by most mammalian cells, is an efficient scavenger of toxic oxidants, including hydrogen peroxide, an oxidant that plays a major role in the oxidant burden placed on the epithelial surface of the lower respiratory tract in chronic inflammatory states. GSH is present in the epithelial lining fluid of the normal lower respiratory tract, where it is thought to play a major role in providing antioxidant protection to the epithelial cells. In this regard, we hypothesized that the lower respiratory tract of patients with IPF may be chronically depleted of this antioxidant, thus leading to an increased susceptibility of lung epithelial cells to oxidant injury. To evaluate this concept, the concentration of glutathione was determined in the epithelial lining fluid of the lower respiratory tract of 15 patients with IPF and compared to that of 19 normal subjects. Strikingly, whereas ELF glutathione concentrations were high in normal subjects (429 +/- 34 microM), a fourfold decrease was found in patients with IPF (97 +/- 18 microM, p less than 0.001). In the context of the known oxidant burden present in the lower respiratory tract of patients with IPF, these observations of a "GSH deficiency" in IPF ELF suggest that there is a marked oxidant-antioxidant imbalance at the alveolar surface of these persons, thus increasing the susceptibility to the severe epithelial cell damage characteristic of this disease.

publication date

  • February 1989

has subject area

  • Adult
  • Antioxidants
  • Bronchoalveolar Lavage Fluid
  • Cell Count
  • Epithelium
  • Female
  • Glutathione
  • Humans
  • Male
  • Middle Aged
  • Oxidation-Reduction
  • Pulmonary Alveoli
  • Pulmonary Fibrosis

Research

keywords

  • Comparative Study
  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1164/ajrccm/139.2.370

PubMed ID

  • 2913886

Additional Document Info

start page

  • 370

end page

  • 372

volume

  • 139

number

  • 2