Construction and properties of replication-competent murine retroviral vectors encoding methotrexate resistance. Academic Article uri icon

Overview

MeSH

  • Animals
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA, Recombinant
  • DNA, Viral
  • Drug Resistance, Microbial
  • Humans
  • Mice
  • Mice, Inbred Strains
  • Mutation
  • Nucleic Acid Conformation
  • Plasmids
  • Tetrahydrofolate Dehydrogenase
  • Transfection
  • Virus Replication

MeSH Major

  • Gene Expression Regulation
  • Genetic Vectors
  • Methotrexate
  • Moloney murine leukemia virus

abstract

  • A series of replication-competent Moloney murine leukemia virus vectors was constructed in which each vector contained a mutant dihydrofolate reductase (DHFR) cDNA insert in the U3 region of the viral long terminal repeat. Two of the resulting viruses, MLV (murine leukemia virus) DHFR*-5 and MLV DHFR*-7, were able to stably transfer methotrexate resistance to infected fibroblast cells upon multiple rounds of virus replication and in the absence of drug selection. Cell lines producing recombinant virus with high titers were established, which indicated that the insert did not grossly interfere with viral replication functions. These vectors should be useful for introducing and expressing foreign genes in vivo in tissues and whole animals in which virus spread is needed for efficient infection.

publication date

  • January 1989

has subject area

  • Animals
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA, Recombinant
  • DNA, Viral
  • Drug Resistance, Microbial
  • Gene Expression Regulation
  • Genetic Vectors
  • Humans
  • Methotrexate
  • Mice
  • Mice, Inbred Strains
  • Moloney murine leukemia virus
  • Mutation
  • Nucleic Acid Conformation
  • Plasmids
  • Tetrahydrofolate Dehydrogenase
  • Transfection
  • Virus Replication

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC362150

PubMed ID

  • 2927389

Additional Document Info

start page

  • 100

end page

  • 108

volume

  • 9

number

  • 1