Activation of yeast RNA polymerase II transcription by a thymidine-rich upstream element in vitro Academic Article uri icon

Overview

MeSH Major

  • RNA Polymerase II
  • Saccharomyces cerevisiae
  • Transcription, Genetic

abstract

  • A thymidine-rich sequence upstream of the DED1 gene of Saccharomyces cerevisiae activated transcription of the CYC1 promoter by RNA polymerase II in vitro. Activation was inhibited by an excess of an oligonucleotide with the same but not a closely related thymidine-rich sequence, pointing to the involvement of a specific thymidine-rich element-binding factor. The extent of activation was as great as 30-fold and showed a similar distance and orientation dependence and a similar effect of deletions in vitro as in vivo.

publication date

  • January 1989

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC286495

PubMed ID

  • 2643115

Additional Document Info

start page

  • 486

end page

  • 90

volume

  • 86

number

  • 2