Coordinate alteration of pp60(c-src) abundance and c-src RNA expression in human neuroblastoma variants Academic Article uri icon

Overview

MeSH Major

  • Neuroblastoma
  • Proto-Oncogene Proteins
  • Proto-Oncogenes
  • RNA, Neoplasm

abstract

  • We examined the expression, abundance, and protein kinase activity of pp60c-src in two different pairs of genetically indistinguishable cloned human neuroblastoma cell variants which display altered phenotypes as the result of conversion from a neuronal to a non-neuronal phenotype. The results demonstrate that cells which exhibit the neuroblastic (N-type) phenotype possess high levels of pp60c-src protein and that one of the two N-type cell lines is capable of expressing the neuronal-specific isoenzyme of pp60c-src. In contrast, cells which display the substrate-adherent (S-type) phenotype have low levels of pp60c-src protein and express exclusively the non-neuronal isoenzyme of pp60c-src. In all cells examined the abundance of pp60c-src was found to be proportional to the steady-state level of c-src RNA. In each case the protein kinase activity of pp60c-src was found to be proportional to the abundance of the protein and independent of the ratio of neuronal to non-neuronal species of pp60c-src.

publication date

  • January 1989

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 2470006

Additional Document Info

start page

  • 421

end page

  • 7

volume

  • 4

number

  • 4