Positive and negative regulation of gene transcription by a retinoic acid-thyroid hormone receptor heterodimer. Academic Article uri icon

Overview

MeSH

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Chromosome Deletion
  • DNA
  • DNA-Binding Proteins
  • Humans
  • Macromolecular Substances
  • Molecular Sequence Data
  • Mutation
  • Oligonucleotide Probes
  • Protein Binding
  • Receptors, Retinoic Acid
  • Transfection
  • Tretinoin

MeSH Major

  • Carrier Proteins
  • Gene Expression Regulation
  • Genes
  • Receptors, Thyroid Hormone
  • Transcription, Genetic

abstract

  • We present evidence that the human thyroid hormone receptor forms a heterodimer with the human retinoic acid receptor. This interaction results in a cooperative increase in binding of the alpha retinoic acid receptor to a subset of thyroid hormone response elements. Mutations within the DNA binding domain or near the C-terminus abolish either receptor's ability to interact cooperatively on these elements. The thyroid hormone-retinoic acid receptor heterodimer exhibits novel transcriptional properties in that coexpression of both receptors at low levels in Green monkey kidney (CV1) cells results in a positive transcriptional effect on promoters containing a palindromic thyroid hormone response element, but has a surprisingly negative effect on a thyroid hormone response element derived from the alpha myosin heavy chain gene. These results suggest that by forming heterodimers, more elab-orate control of transcription can be achieved by creating receptor combinations with differing activities.

publication date

  • November 17, 1989

has subject area

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carrier Proteins
  • Cell Line
  • Chromosome Deletion
  • DNA
  • DNA-Binding Proteins
  • Gene Expression Regulation
  • Genes
  • Humans
  • Macromolecular Substances
  • Molecular Sequence Data
  • Mutation
  • Oligonucleotide Probes
  • Protein Binding
  • Receptors, Retinoic Acid
  • Receptors, Thyroid Hormone
  • Transcription, Genetic
  • Transfection
  • Tretinoin

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 2555064

Additional Document Info

start page

  • 697

end page

  • 708

volume

  • 59

number

  • 4