Serum alpha 1-antitrypsin deficiency associated with the common S-type (Glu264----Val) mutation results from intracellular degradation of alpha 1-antitrypsin prior to secretion. Academic Article uri icon

Overview

MeSH

  • Alleles
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Genes
  • Glutamic Acid
  • Homozygote
  • Humans
  • Mice
  • Molecular Sequence Data
  • Monocytes
  • Plasmids
  • RNA, Messenger
  • Transcription, Genetic
  • Transfection
  • alpha 1-Antitrypsin Deficiency

MeSH Major

  • Glutamates
  • Mutation
  • Valine
  • alpha 1-Antitrypsin

abstract

  • The S-type alpha 1-antitrypsin (alpha 1AT) deficiency allele differs from the normal M1(Val213) allele by a single amino acid substitution (Glu264----Val). To evaluate the molecular pathophysiology responsible for the reduced serum levels of alpha 1AT associated with the S-type allele, alpha 1AT gene expression was examined in blood monocytes, cells which normally produce alpha 1AT, as well as murine fibroblasts modified by retroviral gene transfer to express the S-type and normal M-type human alpha 1AT genes. Northern analysis and S1 protection analysis demonstrated that monocytes of M and S homozygotes both express 1.8-kilobase alpha 1AT mRNA transcripts in comparable levels and similar in structure. Pulse-chase labeling studies demonstrated that both M and S monocytes synthesized and secreted a 52-kDa protein, but the S monocytes secreted significantly less. The cellular lysates of both M and S monocytes contained a newly synthesized 50-kDa precursor form of alpha 1AT, but the S monocytes contained reduced amounts. Pulse-chase labeling in the presence of tunicamycin, an inhibitor of core oligosaccharide addition, demonstrated that S monocytes exhibited a selective inhibition of secretion of 45-kDa nonglycosylated alpha 1AT not observed in M monocytes. Consistent with these observations, murine fibroblasts modified by retroviral gene transfer to contain an integrated human S-type alpha 1AT cDNA demonstrated reduced secretion of alpha 1AT compared with fibroblasts containing an integrated human M-type alpha 1AT cDNA and also reproduced the abnormality of alpha 1AT biosynthesis observed with S-type monocytes. Furthermore, in the presence of leupeptin, an inhibitor of cellular proteinases, the S-type modified fibroblasts demonstrated a selective augmentation of human alpha 1AT secretion not observed for the M-type. Together, these observations are consistent with the concept that the single A----T mutation of the S-type alpha 1AT gene results in reduced cellular secretion of alpha 1AT because the newly synthesized S-type alpha 1AT protein is degraded intracellularly prior to secretion.

publication date

  • June 25, 1989

has subject area

  • Alleles
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Genes
  • Glutamates
  • Glutamic Acid
  • Homozygote
  • Humans
  • Mice
  • Molecular Sequence Data
  • Monocytes
  • Mutation
  • Plasmids
  • RNA, Messenger
  • Transcription, Genetic
  • Transfection
  • Valine
  • alpha 1-Antitrypsin
  • alpha 1-Antitrypsin Deficiency

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 2567291

Additional Document Info

start page

  • 10477

end page

  • 10486

volume

  • 264

number

  • 18