Rapid increase in enzyme and peptide mRNA in sympathetic ganglia after electrical stimulation in humans Academic Article uri icon


MeSH Major

  • Dopamine beta-Hydroxylase
  • Ganglia, Sympathetic
  • Neuropeptide Y
  • RNA, Messenger
  • Transcription, Genetic
  • Tyrosine 3-Monooxygenase


  • Thoracic ganglia in humans were studied after electrical, preganglionic stimulation using in situ hybridization with synthetic oligonucleotide probes against the catecholamine-synthesizing enzymes tyrosine hydroxylase (EC and dopamine beta-hydroxylase (EC and neuropeptide tyrosine. Immunohistochemical analysis was also performed. Following short peroperative stimulation a severalfold increase in all three mRNAs was found in principal ganglion cells, whereas no definite changes could be detected in enzyme or peptide levels with immunohistochemistry. The results suggest a very rapid and sensitive regulation of genes involved in signal transmission in the sympathetic nervous system of humans. Moreover, they indicate that electrical stimulation of neurons and/or pathways combined with in situ hybridization may be used as a method to define neuronal projections by visualizing increases in mRNAs for transmitter enzymes and/or peptide in target cells.

publication date

  • January 1989



  • Academic Article



  • eng

PubMed Central ID

  • PMC287440

PubMed ID

  • 2567003

Additional Document Info

start page

  • 4302

end page

  • 5


  • 86


  • 11