Inositol 1,4,5-trisphosphate receptor localized to endoplasmic reticulum in cerebellar Purkinje neurons Academic Article uri icon


MeSH Major

  • Calcium Channels
  • Cerebellum
  • Endoplasmic Reticulum
  • Heart Conduction System
  • Inositol Phosphates
  • Neurons
  • Purkinje Fibers
  • Receptors, Cell Surface
  • Receptors, Cytoplasmic and Nuclear
  • Sugar Phosphates


  • Inositol 1,4,5-trisphosphate (InsP3) mediates the effects of several neurotransmitters, hormones and growth factors by mobilizing Ca2+ from a vesicular, non-mitochondrial intracellular store. Many studies have indirectly suggested the endoplasmic reticulum (ER) to be the site of InsP3 action, though some have implicated the plasma membrane or a newly described smooth surfaced structure, termed the calciosome. Using antibodies directed against a purified InsP3-receptor glycoprotein, of relative molecular mass 260,000, in electron microscope immunocytochemical studies of rat cerebellar Purkinje cells, we have now localized the InsP3 receptor to ER, including portions of the rough endoplasmic reticulum, a population of smooth-membrane-bound organelles (smooth ER), a portion of subplasmalemmal cisternae and the nuclear membrane, but not to mitochondria or the cell membrane. These results suggest that in cerebellar Purkinje cells, InsP3-induced intracellular calcium release is not the property of a single organelle, but is effected by specialized portions of both rough and smooth ER, and possibly by other smooth surfaced structures. The present findings are the first immunocytochemical demonstration of an InsP3 receptor within a cell.

publication date

  • December 1989



  • Academic Article



  • eng

PubMed ID

  • 2542801

Additional Document Info

start page

  • 468

end page

  • 70


  • 339


  • 6224