Insulin-like Growth Factor II mRNA Expression in Human Breast Cancer Academic Article uri icon

Overview

MeSH Major

  • Breast Neoplasms
  • Insulin-Like Growth Factor II
  • RNA, Messenger
  • Somatomedins

abstract

  • Insulin-like growth factor II is a growth factor important in fetal development. Several cancer tissues and cell lines have been reported to express IGF-II and rat IGF-II is mitogenic for breast cancer cell lines. Using Northern analysis and ribonuclease protection assays, IGF-II mRNA was detected in normal fibroblasts and in the established breast cancer cell line, T47D. In this cell line, steady state levels of IGF-II message were increased by treatment with estradiol. 10 nM IGF-II, purified from human serum, was mitogenic for breast cancer cell lines. In vitro, IGF-II may act as an autocrine growth factor for some cell lines. RNA derived from breast cancer, pathologically normal breast tissue, and benign breast disease also contained IGF-II mRNA. When paired samples of normal and cancer tissue were obtained from the breast of the same patient, the level of IGF-II mRNA expression in the normal tissue was at least that found in the cancer. This is consistent with previous observations that show IGF-II is expressed in mesenchyme. These findings suggest that in breast cancer IGF-II is produced by stromal tissue elements and potentially by the malignant epithelial cells. Therefore, IGF-II may function as an autocrine or a paracrine growth factor in different breast tumors.

publication date

  • January 1988

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 3180080

Additional Document Info

start page

  • 6691

end page

  • 6

volume

  • 48

number

  • 23