A molecular defect in virally transformed muscle cells that cannot cluster acetylcholine receptors Academic Article uri icon

Overview

MeSH Major

  • Cell Transformation, Viral
  • Muscles
  • Receptors, Cholinergic
  • Tropomyosin

abstract

  • Muscle cells infected at the permissive temperature with temperature-sensitive mutants of Rous sarcoma virus and shifted to the non-permissive temperature form myotubes that are unable to cluster acetylcholine receptors (Anthony, D. T., S. M. Schuetze, and L. L. Rubin. 1984. Proc. Natl. Acad. Sci. USA. 81:2265-2269). Work described in this paper demonstrates that the virally-infected cells are missing a 37-kD peptide which reacts with an anti-tropomyosin antiserum. Using a monoclonal antibody specific for the missing peptide, we show that this tropomyosin is absent from fibroblasts and is distinct from smooth muscle tropomyosins. It is also different from the two previously identified striated muscle myofibrillar tropomyosins (alpha and beta). We suggest that, in normal muscle, this novel, non-myofibrillar, tropomyosin-like molecule is an important component of a cytoskeletal network necessary for cluster formation.

publication date

  • January 1988

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2115064

PubMed ID

  • 2836437

Additional Document Info

start page

  • 1713

end page

  • 21

volume

  • 106

number

  • 5