Cloning and characterization of an alpha 1-antitrypsin like gene 12 KB downstream of the genuine alpha 1-antitrypsin gene. Academic Article uri icon

Overview

MeSH

  • Animals
  • Base Sequence
  • Cell Line
  • Chromosome Deletion
  • Chromosome Mapping
  • DNA Restriction Enzymes
  • Molecular Sequence Data
  • Polymorphism, Restriction Fragment Length

MeSH Major

  • Cloning, Molecular
  • Deoxyribonucleases, Type II Site-Specific
  • alpha 1-Antitrypsin

abstract

  • Cosmid clones containing alpha 1-antitrypsin (alpha 1AT) gene sequences were observed to contain alpha 1AT-like sequences approximately 12 kb downstream of the authentic alpha 1AT gene. Restriction mapping suggested the alpha 1AT-like gene lacks promoter sequences. Cosmid clones from one library contained a truncated alpha 1AT-like gene with a deletion encompassing 1745 bp, including the whole exon IV and part of exon V. Sequencing of exon II of this truncated gene revealed a nucleotide homology of 76% but included critical mutations in the start codon (ATG - greater than ATA) and the 3' exon-intron junction. These results strongly suggest that the truncated alpha 1AT-like gene is a pseudogene, which is present at a frequency of 0.30 in the Dutch population.

publication date

  • September 15, 1988

has subject area

  • Animals
  • Base Sequence
  • Cell Line
  • Chromosome Deletion
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA Restriction Enzymes
  • Deoxyribonucleases, Type II Site-Specific
  • Molecular Sequence Data
  • Polymorphism, Restriction Fragment Length
  • alpha 1-Antitrypsin

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 2901833

Additional Document Info

start page

  • 634

end page

  • 642

volume

  • 155

number

  • 2