X-ray Laue diffraction from crystals of xylose isomerase. Academic Article uri icon

Overview

MeSH Major

  • Aldose-Ketose Isomerases
  • Carbohydrate Epimerases

abstract

  • The Laue method (stationary crystal, polychromatic x-rays) was used to collect native and heavy-atom-derivative data on crystals of xylose isomerase (EC 5.3.1.5). These data were used to find the heavy-atom positions. The positions found by use of Laue data are the same as those found by use of monochromatic data collected on a diffractometer. These results confirm that Laue diffraction data sets, which can be obtained on a millisecond time scale, can be used to locate small molecules bound to protein active sites. The successful determination of heavy-atom positions also indicates that x-ray crystallographic data collected by the Laue method can be used to solve protein structures.

publication date

  • January 1988

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC279493

PubMed ID

  • 3422408

Additional Document Info

start page

  • 112

end page

  • 5

volume

  • 85

number

  • 1