Bias toward use of a specific T cell receptor beta-chain variable region in a subgroup of individuals with sarcoidosis. Academic Article uri icon

Overview

MeSH

  • Antibodies, Monoclonal
  • Antigens, Surface
  • Clone Cells
  • Epitopes
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
  • Humans
  • Lung Diseases
  • RNA, Messenger

MeSH Major

  • Immunoglobulin Variable Region
  • Receptors, Antigen, T-Cell
  • Sarcoidosis
  • T-Lymphocytes

abstract

  • To evaluate the concept that biases in the usage of T cell antigen receptor beta variable (V) regions may be manifested in T lymphocytes that accumulate in nonmalignant, T cell-mediated human disorders, a V beta 8-specific antibody (anti-Ti3A, 5REX9H5) was used to evaluate lung and blood T cells in pulmonary sarcoidosis, a chronic granulomatous disorder of unknown etiology. Whereas normal patients had less than 5% Ti3A+ lung (n = 7) and/or blood (n = 9) lymphocytes, strikingly, a subgroup (8 of 21) with active pulmonary sarcoidosis had greater than 7% Ti3A+ lung and/or blood T cells and a higher proportion of Ti3A+ lymphocytes in the lung compared with blood. Dual-color flow cytometry demonstrated compartmentalization of Ti3A+ CD4+ lymphocytes to lung and Ti3A+ CD8+ lymphocytes to blood. Analysis with a 32P-labeled V beta 8 probe revealed that sarcoid lung T lymphocytes contained higher amounts of V beta 8+ mRNA than autologous blood T cells. However, Southern analysis of sarcoid lung and blood T cell DNA demonstrated no evidence of clonal rearrangements of V beta 8 genes. These observations demonstrate a clear bias toward the use of at least one V beta region in sarcoidosis, and suggests T cells accumulate secondary to external selective pressure, rather than in a random polyclonal fashion or by clonal expansion of one or few T cell clones.

publication date

  • October 1988

has subject area

  • Antibodies, Monoclonal
  • Antigens, Surface
  • Clone Cells
  • Epitopes
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
  • Humans
  • Immunoglobulin Variable Region
  • Lung Diseases
  • RNA, Messenger
  • Receptors, Antigen, T-Cell
  • Sarcoidosis
  • T-Lymphocytes

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC442668

Digital Object Identifier (DOI)

  • 10.1172/JCI113715

PubMed ID

  • 2459159

Additional Document Info

start page

  • 1183

end page

  • 1191

volume

  • 82

number

  • 4