Isolation and partial characterization of plasma membrane fatty acid binding proteins from myocardium and adipose tissue and their relationship to analogous proteins in liver and gut Academic Article Article uri icon

Overview

MeSH Major

  • Algorithms
  • Heart Ventricles
  • Hypertrophy, Left Ventricular
  • Image Processing, Computer-Assisted
  • Magnetic Resonance Imaging
  • Ventricular Remodeling

abstract

  • We describe a general method for isolating a class of 40 kDa plasma membrane fatty acid binding proteins which have been identified previously only in rat liver and jejunum. Proteins extracted with 2 M salt from rat adipocyte and cardiac myocyte plasma membranes were subjected to preparative isoelectric focusing. Fractions with pI's ≥ 9.0 were further purified by oleate-agarose affinity chromatography and HPLC. Each tissue yielded a single 40 kDa protein which co-chromatographed with [3h]-oleate on gel permeation HPLC, and reacted on Western blots with an antibody to the corresponding hepatic membrane protein. Related plasma membrane fatty acid binding proteins have now been isolated from each of the major sites of fatty acid transport. © 1987 Academic Press, Inc.

publication date

  • November 13, 1987

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1016/S0006-291X(87)80283-8

PubMed ID

  • 3120716

Additional Document Info

start page

  • 1370

end page

  • 1376

volume

  • 148

number

  • 3