Apolipoprotein B-48 is the product of a messenger RNA with an organ-specific in-frame stop codon Academic Article uri icon

Overview

MeSH Major

  • Apolipoproteins B
  • Codon
  • RNA, Messenger

abstract

  • The primary structure of human apolipoprotein (apo) B-48 has been deduced and shown by a combination of DNA excess hybridization, sequencing of tryptic peptides, cloned complementary DNAs, and intestinal messenger RNAs (mRNAs) to be the product of an intestinal mRNA with an in-frame UAA stop codon resulting from a C to U change in the codon CAA encoding Gln2153 in apoB-100 mRNA. The carboxyl-terminal Ile2152 of apoB-48 purified from chylous ascites fluid has apparently been cleaved from the initial translation product, leaving Met2151 as the new carboxyl-terminus. These data indicate that approximately 85% of the intestinal mRNAs terminate within approximately 0.1 to 1.0 kilobase downstream from the stop codon. The other approximately 15% have lengths similar to hepatic apoB-100 mRNA even though they have the same in-frame stop codon. The organ-specific introduction of a stop codon to a mRNA appears unprecedented and might have implications for cryptic polyadenylation signal recognition and RNA processing.

publication date

  • December 1987

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 3659919

Additional Document Info

start page

  • 363

end page

  • 6

volume

  • 238

number

  • 4825