Membrane biochemistry of the ouabain-resistant potassium transport system Academic Article uri icon

Overview

MeSH Major

  • 4-Nitrophenylphosphatase
  • Amiloride
  • Cell Membrane
  • Ouabain
  • Phosphoric Monoester Hydrolases
  • Potassium

abstract

  • A 6.5-kilobase fragment of genomic DNA from mutant mouse cells under ouabain selection pressure conferred ouabain resistance when transfected into ouabain-sensitive CV1 green monkey fibroblasts. Ouabain resistance was induced in the presence of 10 microM ouabain. Amiloride (500 microM) completely blocked ouabain-insensitive 86Rb+ uptake into these cells. Plasma membranes from these cells demonstrated little sodium-dependent adenosine triphosphatase (ATPase) activity but had potassium-dependent and ouabain-resistant p-nitrophenylphosphatase activity. Like Na+,K+-ATPase this activity was vanadate- and sodium-inhibitable. Also, like the Na+,K+-ATPase, sodium inhibition of the p-nitrophenylphosphatase was reversed by 10 microM adenosine 5'-triphosphate.

publication date

  • December 1987

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 2824373

Additional Document Info

start page

  • I93

end page

  • 4

volume

  • 10

number

  • 5 II SUPPl.