Accurate initiation at RNA polymerase II promoters in extracts from Saccharomyces cerevisiae. Academic Article uri icon

Overview

MeSH Major

  • Promoter Regions, Genetic
  • RNA Polymerase II
  • Saccharomyces cerevisiae
  • Transcription, Genetic

abstract

  • A yeast nuclear extract supported transcription from the CYC1 and PYK1 promoters. Transcription was initiated in vitro at or near sites used in vivo. Deletion of "TATA" sequences abolished the reaction. alpha-Amanitin (10 micrograms/ml) and chloride (100 mM) were highly inhibitory.

publication date

  • December 1987

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC299646

PubMed ID

  • 3321057

Additional Document Info

start page

  • 8839

end page

  • 43

volume

  • 84

number

  • 24