Clonal gene therapy: transplanted mouse fibroblast clones express human alpha 1-antitrypsin gene in vivo. Academic Article uri icon

Overview

MeSH

  • Animals
  • Cell Line
  • Clone Cells
  • DNA, Recombinant
  • Humans
  • Lung
  • Mice
  • Mice, Nude
  • Peritoneal Cavity
  • Retroviridae

MeSH Major

  • DNA
  • Fibroblasts
  • Transformation, Genetic
  • alpha 1-Antitrypsin

abstract

  • A retroviral vector was used to insert human alpha 1-antitrypsin (alpha 1AT) complementary DNA into the genome of mouse fibroblasts to create a clonal population of mouse fibroblasts secreting human alpha 1AT. After demonstrating that this clone of fibroblasts produced alpha 1AT after more than 100 population doublings in the absence of selection pressure, the clone was transplanted into the peritoneal cavities of nude mice. When the animals were evaluated 4 weeks later, human alpha 1AT was detected in both sera and the epithelial surface of the lungs. The transplanted clone of fibroblasts could be recovered from the peritoneal cavities of those mice and demonstrated to still be producing human alpha 1AT. Thus, even after removal of selective pressure, a single clone of retroviral vector-infected cells that expressed an exogenous gene in vitro, continued to do so in vivo, and when recovered, continued to produce the product of the exogenous gene.

publication date

  • August 14, 1987

has subject area

  • Animals
  • Cell Line
  • Clone Cells
  • DNA
  • DNA, Recombinant
  • Fibroblasts
  • Humans
  • Lung
  • Mice
  • Mice, Nude
  • Peritoneal Cavity
  • Retroviridae
  • Transformation, Genetic
  • alpha 1-Antitrypsin

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 3497452

Additional Document Info

start page

  • 762

end page

  • 764

volume

  • 237

number

  • 4816