A human hepatoma cell line expresses a single-chain form of γ-glutamyl transpeptidase Academic Article uri icon

Overview

MeSH Major

  • Carcinoma, Hepatocellular
  • Isoenzymes
  • Liver Neoplasms
  • gamma-Glutamyltransferase

abstract

  • Mammalian gamma-glutamyl transpeptidases characterized thus far have been shown to be heterodimeric glycoproteins. The two subunits are derived from a single-chain propeptide which, in the rat kidney, exhibits low transpeptidase activity (less than 2% of the dimeric enzyme). A human hepatoma-derived cell line, Hep G2, expresses relatively high transpeptidase activity. The enzyme is primarily localized on the cell surface and exhibits catalytic properties similar to the dimeric human kidney and lymphoid cell transpeptidase. Significantly, the Hep G2 enzyme, unlike the enzyme from other human tissues, is a single-chain species, Mr = 120,000.

publication date

  • January 1987

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 2887558

Additional Document Info

start page

  • 11403

end page

  • 6

volume

  • 262

number

  • 24