In vivo phosphorylation of axonal proteins in goldfish optic nerve during regeneration Academic Article uri icon

Overview

MeSH Major

  • Axons
  • Cyprinidae
  • Goldfish
  • Nerve Regeneration
  • Nerve Tissue Proteins
  • Optic Nerve
  • Phosphoproteins

abstract

  • In vivo phosphorylation of axonal proteins was investigated in normal and regenerating optic nerves of goldfish by two-dimensional gel electrophoresis. By 6-24 h after intraocular injection of H3(32)PO4, approximately 20 optic nerve proteins ranging in size from 19 to 180 kilodaltons and in pI from 4.4 to 6.8 were seen to have incorporated radiolabel. Five of these proteins showed a robust increase in incorporation of phosphate during regeneration. Among the latter was an acidic (pI 4.5) 45-kilodalton protein, which has previously been shown to be conveyed by fast axonal transport and to increase dramatically in its rate of synthesis during regeneration of goldfish optic axons.

publication date

  • January 1987

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 2432175

Additional Document Info

start page

  • 279

end page

  • 83

volume

  • 48

number

  • 1