Generation of human B-cell hybridomas secreting monoclonal anti-myelin-associated glycoprotein antibodies from a patient with neuropathy Academic Article uri icon

Overview

MeSH Major

  • Antibodies, Monoclonal
  • B-Lymphocytes
  • Hybridomas
  • Myelin Proteins
  • Peripheral Nervous System Diseases

abstract

  • Human hybridomas that secrete monoclonal IgM anti-myelin-associated glycoprotein antibodies were generated by fusion with cells from a patient with peripheral neuropathy and IgM monoclonal gammopathy. Karyotypic analysis of the hybridoma cells revealed no chromosomal abnormalities. The cells were positive for cell-surface idiotype HLA-DR and the plasma cell antigen PCA-1, and negative for the B-cell determinant B4 and for Leu-1, which has been postulated to distinguish a subpopulation of B cells that secrete IgM with autoantibody activity.

publication date

  • April 13, 1987

Research

keywords

  • Academic Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1002/ana.410210217

PubMed ID

  • 2435225

Additional Document Info

start page

  • 207

end page

  • 11

volume

  • 21

number

  • 2