Inhibition of secretion of hepatitis B surface antigen by a related presurface polypeptide Academic Article uri icon

Overview

MeSH Major

  • Hepatitis B Surface Antigens
  • Protein Precursors

abstract

  • The presurface (preS) proteins of hepatitis B virus are structural components of the viral envelope that may play important roles in virion assembly and infectivity. They are specified by a large open reading frame that includes the coding region for the major surface (S) protein in its 3' half. Translation of the preS proteins initiates upstream from the S region, giving rise to proteins that are composed of the S domain and an additional 163 (preS1) or 55 (preS2) amino acids. Little is known about the biosynthesis and assembly of these proteins. The expression of the S and preS1 proteins was examined by transfecting cultured mammalian cells with viral DNA and injecting synthetic messenger RNA's into Xenopus oocytes. In contrast to the proteins encoded by the S region, the preS1 proteins are not detectably secreted into the culture medium. Furthermore, when the S and preS1 proteins are synthesized together, secretion of the S proteins is specifically and strongly inhibited. The results suggest a unique molecular interaction during secretion of the S and preS proteins that may be important for virus assembly.

publication date

  • December 1986

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 3787251

Additional Document Info

start page

  • 1388

end page

  • 91

volume

  • 234

number

  • 4782