Cell cycle-dependent modulation of o-methylguanine-dna methyltransferse in c3h/10t1/2 cells Academic Article Article uri icon

Overview

MeSH Major

  • Gastric Mucosa
  • Gastrointestinal Diseases
  • Intestinal Mucosa
  • Oxidative Stress
  • Reactive Oxygen Species

abstract

  • O6-methylguanine-DNA methyltransferase (MGMT) was measured in partially synchronized cultures of C3H/10T1/2 mouse embryo cells as a function of cell cycle. The degree of synchrony and progression of the cell cycle were monitored by flow cytometry. The MGMT level was significantly reduced prior to the onset of S-phase. This reduction was concomitant with the inhibition of in vivo repair of O6-methylguanine in DNA of S-phase cells as observed earlier. The recovery of the MGMT level paralleled the progression of synchronized cells into G2. S-phase cells purified by cell sorting contained approximately 15% of the MGMT present in G0 or early G1 cells. A comparison of the in vivo repair of O6-methylguanine and MGMT levels suggests that the lack of repair of O6-methylguanine in DNA of the mouse embryo cells is due only in part to a temporal loss of MGMT.

publication date

  • May 1986

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1093/carcin/7.5.807

PubMed ID

  • 3698207

Additional Document Info

start page

  • 807

end page

  • 12

volume

  • 7

number

  • 5