The human apolipoprotein B-100 gene: A highly polymorphic gene that maps to the short arm of chromosome 2 Academic Article Article uri icon

Overview

MeSH Major

  • Obesity
  • Thinness
  • Weight Gain

abstract

  • Using a cloned cDNA of apolipoprotein B-100 as hydridization probe, we have found high frequence polymorphisms in the apoB-100 gene involving sites for the restriction enzymes EcoRI, BamHI, and HindIII. The major EcoRI polymorphisms involved a 17 kb vs 15 kb variant. The incidence of the various phenotypes was estimated. In addition, other complex polymorphisms involving MspI and TaqI sites were also noted. [32P]-labeled apoB-100 cDNA was used as a probe in chromosome mapping studies to detect the human apoB-100 structural gene sequence in human-Chinese hamster and human-mouse cell hybrids. Southern blot analysis of 14 hybrids localized the gene to the short arm of human chromosome 2.

publication date

  • November 27, 1985

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1016/0006-291X(85)91868-6

PubMed ID

  • 4074366

Additional Document Info

start page

  • 248

end page

  • 55

volume

  • 133

number

  • 1