Binding of anti-Z-DNA antibodies in quiescent and activated lymphocytes: Relationship to cell cycle progression and chromatin changes Academic Article uri icon

Overview

MeSH Major

  • Antibodies
  • Chromatin
  • DNA
  • Lymphocyte Activation
  • Lymphocytes

abstract

  • Although regions of DNA reacting with anti-Z-DNA antibodies have been identified in the polytene chromosomes of Drosophila spp. and the metaphase chromosomes from a number of different mammalian species, the biological role of this DNA is unknown. Flow cytometry was used in the present studies to quantitate the binding of anti-Z-DNA antibodies in quiescent and activated human peripheral blood lymphocytes; the antibody binding was then correlated with cell cycle phase. The data show that quiescent (G0 or G1Q) lymphocytes are heterogeneous with respect to their reaction with anti-Z-DNA antibodies. The transition from quiescence (G1Q) into the cell cycle (G1), which involves decondensation of chromatin, did not result in any significant change in binding of these antibodies. In contrast, progression of cells from G1 through S and G2 is correlated with a 27% decrease in anti-Z-DNA antibody reactivity relative to total DNA content. No significant change was observed during the transition from G2 to mitosis (M).

publication date

  • December 1985

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC369143

PubMed ID

  • 3915771

Additional Document Info

start page

  • 3270

end page

  • 3

volume

  • 5

number

  • 11