Anti-MAG antibody and antibody complexes: Detection by radioimmunoassay Academic Article uri icon

Overview

MeSH Major

  • Antibodies, Anti-Idiotypic
  • Myelin Proteins
  • Radioimmunoassay

abstract

  • A radioimmunoassay (RIA) for measuring isotype-specific antibodies to the myelin-associated glycoprotein (MAG) was developed using radiolabeled CNS MAG in a double-antibody precipitation system. Anti-MAG activity was detected by RIA only in patients with neuropathy and anti-MAG M proteins. Anti-MAG IgM or IgG antibodies were not detected in serum of patients with Guillain-BarrĂ© syndrome, chronic relapsing polyneuritis, or multiple sclerosis (MS). Some patients with anti-MAG IgM M proteins also had complexes of IgG or IgA bound to the M protein. In one patient, anti-CNS MAG activity was detected by RIA, but not by ELISA or immunoblot. Anti-MAG antibody activity in patients with neuropathy seems to be isotypically restricted, and there is no evidence for antibody reactivity to MAG in other demyelinating diseases.

publication date

  • January 1985

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 2409476

Additional Document Info

start page

  • 988

end page

  • 92

volume

  • 35

number

  • 7