Local cytoplasmic calcium gradients in living mitotic cells Academic Article Article uri icon


MeSH Major

  • Carrier Proteins
  • Membrane Glycoproteins
  • Membrane Transport Proteins
  • Sterols


  • Cytoplasmic free calcium has been proposed as a regulator of many microtubule-mediated processes, including mitosis. It has been difficult to test this hypothesis because methods for local measurement of free Ca2+ in the living cell have not been available. We have used the fluorescent calcium indicator dye Quin-2 (methoxyquinoline-1bis(o-aminophenoxy)ethane-N,N,N',N' -tetra acetic acid), which allows such observations to be made by digital processing of fluorescent images from the light microscope. Here we report the application of this technique to the study of local Ca2+ concentrations in mitotic endosperm cells of Haemanthus sp., and show that there is transient increase in free Ca2+ at the mitotic spindle poles during anaphase. This locally high Ca2+ may provide a mechanism for the regional control of microtubules and other cytoskeletal elements during anaphase.

publication date

  • October 31, 1985



  • Academic Article


Digital Object Identifier (DOI)

  • 10.1038/316848a0

PubMed ID

  • 4033782

Additional Document Info

start page

  • 848

end page

  • 50


  • 316


  • 6031