Introduction of a selectable gene into different animal tissue by a retrovirus recombinant vector. Academic Article uri icon

Overview

MeSH

  • Animals
  • Base Sequence
  • DNA, Viral
  • Gene Expression Regulation
  • Genes, Bacterial
  • Genes, Viral
  • Gestational Age
  • Moloney murine leukemia virus
  • Moloney murine sarcoma virus

MeSH Major

  • Genetic Vectors
  • Mice
  • Retroviridae

abstract

  • The potential use of retrovirus vectors to transduce foreign genetic information into cells of different tissues of an animal was explored by introducing a recombinant genome carrying the Eco gpt gene into postimplantation mouse embryos. To obviate the need for preparing concentrated virus stocks, psi 2-2-5 cells producing the replication-defective murine sarcoma virus (MSV)-gpt virus were microinjected directly into embryos. The psi 2-2-5 cells were mixed with cells producing replication-competent Moloney murine leukemia virus (Mo-MuLV) to facilitate spread of the vector. A high percentage of the manipulated embryos continued to develop without disturbance and were analyzed either prior to birth or as adults for expression of both helper and Eco gpt virus. Microinjection of as few as 10 Mo-MuLV-producing cells resulted in viremia of greater than 50% of the embryos or adults, 25%-30% of which produced MSV-gpt recombinant virus in a variety of organs including thymus, spleen, lung, kidney, and brain. The fraction of vector-producing cells, however, was 3 to 5 orders of magnitude lower than that of helper-virus-producing cells. Our results demonstrate that a selectable gene can be introduced by retroviral vectors into animals and can be expressed in a wide variety of different somatic tissues.

publication date

  • November 1984

has subject area

  • Animals
  • Base Sequence
  • DNA, Viral
  • Gene Expression Regulation
  • Genes, Bacterial
  • Genes, Viral
  • Genetic Vectors
  • Gestational Age
  • Mice
  • Moloney murine leukemia virus
  • Moloney murine sarcoma virus
  • Retroviridae

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC392095

PubMed ID

  • 6095271

Additional Document Info

start page

  • 7151

end page

  • 7155

volume

  • 81

number

  • 22