Small oligomers of immunoglobulin E (IgE) cause large-scale clustering of IgE receptors on the surface of rat basophilic leukemia cells. Academic Article uri icon

Overview

MeSH

  • Animals
  • Cell Line
  • Cell Membrane
  • Fluorescent Dyes
  • Microscopy, Fluorescence
  • Photography
  • Rats
  • Receptors, IgE

MeSH Major

  • Basophils
  • Immunoglobulin E
  • Leukemia, Experimental
  • Peptide Fragments
  • Receptors, Immunologic

abstract

  • We examined the distribution of small oligomers of IgE bound to rat basophilic leukemia cells using fluorescence microscopy. The oligomers were seen to cluster into visible patches on the cell surface at 4 degrees C; at higher temperatures internalization also was observed. In contrast, cells labeled with IgE monomers remained predominantly ring-stained. Evidence is provided that the observed clustering of IgE oligomers is a cell-induced phenomenon, and the possible significance of this clustering is discussed in the context of the oligomer-triggered degranulation of rat basophilic leukemia cells.

publication date

  • February 1984

has subject area

  • Animals
  • Basophils
  • Cell Line
  • Cell Membrane
  • Fluorescent Dyes
  • Immunoglobulin E
  • Leukemia, Experimental
  • Microscopy, Fluorescence
  • Peptide Fragments
  • Photography
  • Rats
  • Receptors, IgE
  • Receptors, Immunologic

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2113094

PubMed ID

  • 6229545

Additional Document Info

start page

  • 577

end page

  • 583

volume

  • 98

number

  • 2