Transformation by Rous sarcoma virus prevents acetylcholine receptor clustering on cultured chicken muscle fibers Academic Article uri icon

Overview

MeSH Major

  • Avian Sarcoma Viruses
  • Cell Transformation, Neoplastic
  • Muscles
  • Receptors, Cholinergic

abstract

  • Acetylcholine receptors aggregate in the membrane of cultured chicken myotubes; the process of receptor clustering can be stimulated by exogenous factors that we, among others, have begun to characterize. Chicken myoblasts transformed by temperature-sensitive mutants of Rous sarcoma virus, such as tsNY68, fuse to form multinucleated myotubes at 42 degrees C, the nonpermissive temperature for transformation. However, tsNY68-infected myotubes do not cluster acetylcholine receptors at 42 degrees C, even in the presence of active clustering agents. This phenomenon is not merely a result of viral infection, since myotubes infected with a transformation-deficient viral mutant, td107A, behave like noninfected myotubes with respect to receptor clustering; thus, the effects of tsNY68 on the clustering process must be mediated by the src gene product. These experiments may provide a method of identifying essential elements of acetylcholine receptor clusters.

publication date

  • January 1984

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC345479

PubMed ID

  • 6326117

Additional Document Info

start page

  • 2265

end page

  • 9

volume

  • 81

number

  • 7 I