Regional distribution of metorphamide in rat and guinea pig brain Academic Article Article uri icon


MeSH Major

  • Graft Rejection
  • Kidney Transplantation
  • Primary Graft Dysfunction
  • RNA, Messenger


  • A specific radioimmunoassay was developed for metorphamide, an endogenous, amidated opioid octapeptide, originally isolated from bovine brain and human pheochromocytoma tissues. The radioimmunoassay was used to determine the concentration of immunoreactive metorphamide in extracts from dissected regions of rat and guinea pig brain. Radioimmunoassay interfacing with Sephadex gel filtration and reverse phase high performance liquid chromatography confirmed that the immunoreactive substance measured corresponded to authentic metorphamide. Metorphamide was found to be widely distributed in brain regions from both species. However, the concentrations of immunoreactive metorphamide in regions from guinea pig brain were up to 5 times higher than the concentrations of immunoreactive metorphamide in rat brain regions. The results suggest that metorphamide is a specific processing product from proenkephalin in rodent brain.

publication date

  • August 16, 1984



  • Academic Article


Digital Object Identifier (DOI)

  • 10.1016/0006-291X(84)91174-4

PubMed ID

  • 6477570

Additional Document Info

start page

  • 892

end page

  • 8


  • 122


  • 3