Directional transport and integration of donor DNA in Haemophilus influenzae transformation Academic Article uri icon

Overview

MeSH Major

  • DNA, Bacterial
  • Haemophilus influenzae
  • Transformation, Bacterial

abstract

  • DNA transport and integration in Haemophilus influenzae transformation was studied with a plasmid clone of homologous DNA (pCML6). Our results indicate that: (i) donor DNA enters specialized membranous extensions on the cell surface, which we have termed "transformasomes"; (ii) linear DNA undergoes degradation upon exiting transformasomes; and (iii) DNA without a free end remains within transformasomes and is not degraded. By comparing the fate of label from uniformly labeled versus middle-labeled DNA, it appears that donor DNA undergoes degradation from an end prior to recombining with the chromosome. Using donor DNA with covalently closed termini (hairpin ends) prevents efficient exit from transformasomes. When one hairpin is removed, exit of donor DNA is shown to be directional from the free end, with preferential homologous integration of the 3' strand from that end.

publication date

  • December 1983

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC390037

PubMed ID

  • 6316352

Additional Document Info

start page

  • 7274

end page

  • 8

volume

  • 80

number

  • 23 I