Reduction of interference by specific IgG with a modified microtiter solid-phase radioimmunoassay to measure honeybee venom IgE Academic Article Article uri icon

Overview

MeSH Major

  • Antibodies, Bispecific
  • Antigens, Neoplasm
  • Antineoplastic Agents
  • Carcinoma, Hepatocellular
  • Immunotherapy
  • Liver Neoplasms
  • Receptors, Antigen
  • Recombinant Fusion Proteins

abstract

  • By means of monoclonal mouse anti-human IgE antibody, the microtiter solid-phase radioimmunoassay (MSPRIA) was modified to measure honeybee venom (HBV) IgE. HBV IgG was measured by MSPRIA(G) using affinity column purified goat anti-human IgG antibody and correlated with results obtained with staphylococcal protein A-SPRIA. Nonlinear specific IgE dilution curves were associated with high HBV IgG content. By serial transfer and assay of the supernatant, the interference of IgE by specific IgG could be reduced. The degree of interference was correlated with the specific IgG content. The principle of serial supernatant transfer and assay was applied to the standardization of reference HBV IgE serum, and the quantitation of HBV IgE contents in patients and controls. HBV IgE levels in patients allergic to honeybee stings correlated with the skin-test endpoint titrations. While 75% of the allergic patients were skin-test positive, 83% of them were IgE positive by the MSPRIA and 67% were IgE positive by the RAST.

publication date

  • January 1983

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1016/0091-6749(83)90082-9

PubMed ID

  • 6600761

Additional Document Info

start page

  • 283

end page

  • 93

volume

  • 71

number

  • 3