Purification of a progesterone receptor from rabbit uterus Academic Article uri icon

Overview

MeSH Major

  • Receptors, Progesterone
  • Uterus

abstract

  • A progesterone receptor has been purified to homogeneity from rabbit uterus by steroid affinity chromatography. The receptor was obtained in 5% yield, with a specific activity for [ 3 H]progesterone binding of 14,580 pmol/mg protein. The pure receptor migrated as a single band on SDS-polyacrylamide electrophoresis, with a MW of 70,000. Progesterone binding to the receptor was heat labile and was displaced by an excess of R5020. Photoaffinity labeling of the pure receptor with [ 3 H]R5020 corresponded to the major photoaffinity labeled species in crude cytosol. © 1982.

publication date

  • October 15, 1982

Research

keywords

  • Academic Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1016/0006-291X(82)92118-0

PubMed ID

  • 6891211

Additional Document Info

start page

  • 1131

end page

  • 5

volume

  • 108

number

  • 3