Measurement of myelin basic protein and of anti-basic protein antibodies by ELISA utilizing biotinylated antibodies Academic Article uri icon

Overview

MeSH Major

  • Autoantibodies
  • Myelin Basic Protein
  • Succinimides

abstract

  • Immunoglobulins were conjugated to peroxidase by the biotin-avidin method and used in ELISA systems for measuring myelin basic protein (MBP) and anti-MBP antibodies. To measure concentration of MBP, microplate wells were coated with affinity purified rabbit anti-MBP antibodies and incubated with varying concentrations of MBP. Bound antigen was measured by incubating with biotinylated anti-MBP antibodies and avidin-peroxidase. As little as 0.2 ng/ml of MBP could be measured by this assay. To measure anti-MBP antibodies, microplate wells were coated with human MBP and incubated with varying concentrations of affinity purified rabbit anti-human MBP antibodies. Binding was measured by incubating with either peroxidase-conjugated anti-rabbit antibodies or biotinylated anti-rabbit antibodies and avidin peroxidase. The two methods were equally sensitive. The avidin-biotin method for enzyme conjugation promises to be a useful and versatile tool for ELISA systems.

publication date

  • May 26, 1983

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 6194103

Additional Document Info

start page

  • 31

end page

  • 7

volume

  • 12

number

  • 1