Mutagenesis of bacteriophage T7 in vitro by incorporation of O6-methylguanine during DNA synthesis Academic Article uri icon

Overview

MeSH Major

  • DNA Replication
  • Guanine
  • Mutation
  • T-Phages

abstract

  • An in vitro system in which bacteriophage T7 DNA is replicated and efficiently packaged into procapsids to form viable phage has been used to examine mutagenesis. The fidelity of replication was assayed both by measuring reversion of an amber mutation in an essential gene and by generation of temperature-sensitive mutants among the phage produced in vitro. Under standard reaction conditions, the fidelity of DNA replication is about equal to that normally found in vivo. However, when O6-methyldeoxyguanosine triphosphate is included in the reaction, O6-methylguanine is incorporated into newly synthesized DNA and the mutation frequencies increase 10- to 70-fold over the control. These experiments demonstrate in vitro mutagenesis with the T7 DNA replication-packaging system and provide more direct evidence for the premutagenic role of O6-methylguanine.

publication date

  • December 1982

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC347355

PubMed ID

  • 6961422

Additional Document Info

start page

  • 7440

end page

  • 4

volume

  • 79

number

  • 23 I