O6-Methylguanine-DNA methyltransferase in wild-type and ada mutants of Escherichia coli Academic Article uri icon

Overview

MeSH Major

  • Escherichia coli
  • Methyltransferases

abstract

  • O(6)-Methylguanine-DNA methyltransferase is induced in Escherichia coli during growth in low levels of N-methyl-N'-nitro-N-nitrosoguanidine. We have developed a sensitive assay for quantitating low levels of this activity with a synthetic DNA substrate containing 3H-labeled O(6)-methylguanine as the only modified base. Although both wild-type and adaptation-deficient (ada) mutants of E. coli contained low but comparable numbers (from 13 to 60) of the enzyme molecules per cell, adaptation treatment caused a significant increase of the enzyme in the wild type but not in the ada mutants, suggesting that the ada mutation is in a regulatory locus and not in the structural gene for the methyltransferase.

publication date

  • December 1982

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC221460

PubMed ID

  • 6749819

Additional Document Info

start page

  • 534

end page

  • 7

volume

  • 152

number

  • 1